After a private investigator’s alarming revelation that there exists long haul truckers moonlighting as serial killers terrorizing America’s interstate system, Josh and Rachel begin a cross-country journey to research these killers on wheels. While hitchhiking, the filmmakers encounter women who work truck stops, despairingly referred to as “lot lizards,” and hear first hand accounts of the dangers they experience everyday. As Josh and Rachel delve deeper, they uncover systemic failures in law enforcement allowing these mobile killers to run wild. A disturbing confession by a former trucker, serving a life sentence for murder, propels Josh and Rachel on a hunt for his accomplices. When a potential serial killer turns up dead in West Virginia, the investigation takes a surprising turn and we learn that things are not always what they seem.